3M™ Attest™ Rapid Ethylene Oxide Biological Indicator, 1294-S, Green Cap, 4 hr, 300/CAR, 2 CAR/CS, 600/CS

The ISO 11138-1:2017 and ISO 11138-2:2017 compliant 3M™ Attest™ Rapid Ethylene Oxide Biological Indicator, 1294-S, is appropriate for use in industrial (Life Sciences) ethylene oxide (EO, EtO) sterilization applications when verified by the end user. The self-contained biological indicator (BI) consists of a Bacillus atrophaeus spore strip and sealed glass ampoule with growth media. The spore strip and media ampoule are contained in a plastic vial topped with a green color-coded cap. The label includes a chemical process indicator. The BI is packaged in a foil pouch designed to protect the product from humidity excursions.

After the ethylene oxide sterilization process, the BI is activated to join the growth media with the processed spore strip. The BI is incubated in a 3M™ Attest™ 390G Auto-reader for a final fluorescent result at 4 hours. When processed, the chemical process indicator turns from reddish-brown to green.