3M™ Harvest RC

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Simplification of high cell density cell culture fluid clarification unit operations

Remove cells, cell debris and DNA (<500 ppb) from the harvested cell culture fluid

Optimized for PCV high density CHO cell culture (5-8% PCV)

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Tech Data Sheet (PDF, 2MB)
Green background on left and 3M Harvest RC Chromatographic Clarifier BT500 product out of package on right

Efficient workflows. Excellent clarity.

3M™ Harvest RC Chromatographic Clarifier, BT500 is the latest addition to the 3M™ Harvest RC Chromatographic Clarifier portfolio. It's a rapid harvesting solution that saves time and steps without the need for a centrifuge.

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Details

Highlights
  • Simplification of high cell density cell culture fluid clarification unit operations
  • Remove cells, cell debris and DNA (<500 ppb) from the harvested cell culture fluid
  • Optimized for PCV high density CHO cell culture (5-8% PCV)
  • Replacement of primary, secondary and guard membrane clarification stages
  • Typical product recovery of 95+% (capsules)
  • Synthetic chromatographic harvest media with chemically defined extractables
  • Predictable scaling from discovery to manufacturing
  • Lower total cost of manufacturing compared to centrifugation and depth filtration
  • Lower consumption of buffer and water compared to depth filtration
  • Capsules fit into laboratory to manufacturing scale workflows

The 3M™ Harvest RC Chromatographic Clarifier is a single stage single-use chromatographic clarification solution designed as an efficient option for harvesting and clarification of modern cell cultures. Clarification with this chromatographic clarifier offers fewer processing steps, higher yield and lower production time and costs.

 Harvest RC Chromatographic Clarifier: Single-stage chromatographic clarification

3M™ Harvest RC Chromatographic Clarifier

Single-stage chromatographic clarification for recombinant protein therapeutic manufacturing

3M™ Harvest RC Chromatographic Clarifier encapsulates innovative synthetic fibrous anion exchange (AEX) chromatography media and a 0.2 µm polyether sulfone (PES) membrane. This enables a single-stage clarification process of low to high-density cell culture (>40 million cells per mL) with high product recovery and high fidelity of soluble and insoluble contaminant separation. Cells are bound inside the media by electrostatic charge interaction with the AEX chromatographic fibers. This results in the efficient retention of large and small particulates without developing a surface cake layer. The media can also remove soluble impurities which results in cleaner effluent than centrifugation or depth filtration.

Experience 3M innovation from lab to production scale.

  • Harvest RC Chromatographic Clarifier: Discovery phase solutions
    Discovery phase solutions

    Start your discovery process with lab-scale solutions that enable you to do your job in less time with less complexity.

  • Harvest RC Chromatographic Clarifier: Production-scale solutions
    Production-scale solutions

    Advance from lab to production scale predictably with the 3M™ Harvest RC Chromatographic Clarifier portfolio of lab and manufacturing capsules.

Read these peer reviewed publications evaluating 3M™ Harvest RC Chromatographic Clarifier.

  •  Harvest RC Chromatographic Clarifier: Chromatographic capture of cells
    Chromatographic capture of cells to achieve single stage clarification in recombinant protein purification

    3M™ Harvest RC Chromatographic Clarifier compares as a single stage single-use platform for harvest and clarification.

  • Harvest RC Chromatographic Clarifier: Single-stage chromatographic clarification
    Single-stage chromatographic clarification reduces cost of protein production

    3M™ Harvest RC Chromatographic Clarifier intensifies and reduces cost of non-mAb protein production.

Suggested applications
  • Biopharmaceutical processing applications of aqueous based pharmaceutical (drugs) and vaccines in accordance with the product instructions and specifications, and cGMP requirements (for BC340, BC1020, BC2300 and BC16000) or GLP requirements (for CT15, WP6, BC4, BC25 and BT500), where applicable
  • Significant turbidity reduction
  • Single-stage chromatographic clarification

Specifications

Resources

Always follow your user instruction manual.