- Overview
- PRODUCTS
- 3M™ Emphaze™ AEX Hybrid Purifier
- 3M™ Harvest RC Chromatographic Clarifier
- 3M™ Polisher ST
- 3M™ Zeta Plus Depth Filters
- 3M™ LifeASSURE™ Filters
- 3M™ Encapsulated System Holders
- View All Products
- Solutions
- Harvest and Clarification Solutions
- Fiber Chromatographic Clarification Solutions
- Purification and Contaminant Removal Solutions
- View All Solutions
- Applications
- mAb and Recombinant Protein Manufacturing
- Plasma Fractionation
- Gene Therapy
- Vaccines
- Chemical Pharmaceuticals
- Education
- Support
Fiber Chromatographic Clarification
Step into the future with our advanced fiber chromatographic technology to help streamline your process.
Next-generation technology for harvest and clarification
As you work with higher density cell cultures providing higher titers in your biopharmaceutical manufacturing processes, your ability to harvest cell culture consistently and effectively becomes more challenging. 3M’s anion exchange (AEX) single-use fiber chromatographic clarification solutions can help streamline your production process.
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High purity: Lower HCPs and DNA post Protein-A -
Linear scalability from lab to commercial production -
Enhancing Protein-A column performance -
Ease of use: Single-use from early stage to GMP production -
Cleaner effluent: Introducing chromatographic separation earlier to remove soluble impurities
Biopharmaceutical leaders perspective
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Read about challenges and unmet needs with current harvest technologies from leaders in biopharmaceutical manufacturing. As technology developers learn more about these unmet needs, they can introduce new solutions that can bring cost savings that are significant enough to be worth investment and justify implementation.
Reimagine harvest and push the boundaries of clarification with anion-exchange (AEX) fiber chromatography
Clarification is a critical unit operation in the production of mAbs and recombinant proteins because it directly affects yield, product consistency and performance of downstream unit operations. 3M created a novel purification method by filtering with charge rather than by particle size. The media is designed with a synthetic nonwoven material containing quaternary amine grafted fibers, which can remove more impurities than traditional depth filters.
Large contaminants such as cells and cell debris, plus small insoluble and soluble impurities like DNA and HCP, can be removed at this early stage. Enabling a higher purity earlier in the purification process makes downstream processing easier.
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Filtering with charge rather than particle sizeViewed with a scanning electron microscopy (SEM), cells can be seen adsorbed and captured throughout the matrix of the functional nonwoven bed of 3M™ Harvest RC Chromatographic Clarifier.
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Making downstream processing easierAEX fibrous functional media can effectively capture cells, cell debris, DNA and HCP that are negatively charged at the harvest cell culture pH. Therefore, the positively charged molecule of interest can flow through.
See how fiber chromatographic clarification technologies perform
3M offers two fiber chromatographic clarification technologies: 3M™ Harvest RC Chromatographic Clarifier, a single-stage harvest solution, and 3M™ Emphaze™ Hybrid Purifier, a secondary clarification solution. Read about these technologies in the following studies.
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3M™ Harvest RC Chromatographic Clarifier
This study, “Enabling Process Simplification through Chromatographic Clarification with High Titer Cell Culture,” was completed by Thermo Fisher Scientific.
In the study, we illustrated that using the 3M™ Harvest RC could result in highly clean filtrate with a single stage allowing for a simplified process development and process set-up. This single-stage set-up also resulted in improved yield compared to traditional multiple-stage set-up that would translate to product loss.
Read the case study (PDF, 867 KB)
See how 3M™ Harvest RC Chromatographic Clarifier compares as a single-stage single use platform for harvest and clarification.
Read the peer-reviewed paper: Chromatographic capture of cells to achieve single stage clarification in recombinant protein purification.
Continue to learn more about 3M™ Harvest RC Chromatographic Clarifier
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3M™ Emphaze™ AEX Hybrid Purifier
3M collaborated with Polpharma Biologics for this study, “DNA impurity removal at the clarification stage is the key to mAb purification process efficiency improvement.”
This study demonstrated that host cell DNA-related impurities such as chromatin interfere with the protein A capture step and removal before the capture step can significantly increase performance and robustness of Protein A capture chromatography.
3M™ Emphaze™ AEX Hybrid Purifier can address the challenge of the soluble DNA impurities and reduction of host cell proteins (HCPs).
To learn more about the importance of Protein A capture chromatography in mAb purification processes, read: Chromatographic clarification overcomes chromatin-mediated hitch-hiking interactions on Protein A capture column.
Continue to learn more about 3M™ Emphaze™ AEX Hybrid Purifier
Simplify clarification with a truly modern and scalable platform
3M™ Harvest RC Chromatographic Clarifier platform is a high-capacity, synthetic, single-use clarification platform that includes a direct clarification solution and a centrate clarification solution.
Learn how it can help you improve speed to market by streamlining process development and providing consistent and comparable high-quality clarified fluid across the platform.
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Scale with confidence.
Scalable lab devices are an easy way to test new technology with minimal commitment. Our scalable lab devices work across the process train with linear, scalable results – helping you and your team simplify your development process.
Find 3M biopharmaceutical solutions
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Find solutions for process-related impurities leveraging membrane and chromatography technology.
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Find solutions for more efficient bioprocessing across our portfolio.
Explore applications
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One step can transform your mAb and recombinant protein manufacturing process. At 3M, we’re helping build better, more efficient and simpler manufacturing processes by developing solutions to enhance biologic drug recovery that leads to improved process economics.
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Gene therapy is a fast-changing, promising field with new challenges for manufacturing and separation. Learn how we can help with our technologies and expertise.
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Plasma fractionation has existed for decades, but additional separation methods are becoming increasingly common. We offer a range of products to help you with your process.
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Getting the most out of your manufacturing process with speed and on budget is an increasing challenge. Our solutions may help you develop manufacturing processes for vaccines to help maximize purity, leading to overall process economics.
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If you need solutions for catalyst removal and recovery, color removal, particulate filtration and fill-finish, 3M offers a wide range of adsorptive depth filters and membrane products for application in the chemical pharmaceutical manufacturing.
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